Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- OAAB04660 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- EPHA10 antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of EPHA10 (arrow) using rabbit polyclonal EPHA10 Antibody (N-term) either nontransfected (Lane 1) or lysates transiently transfected transfected with the EPHA10 gene (Lane 2) (Origene Technologies).(2ug/ml,)
- Sample type
- lysatestransiently transfected with the EPHA10 gene
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of EPHA10 Antibody (N-term) in K562 cell lysates (35ug/lane). EPHA10 (arrow) was detected using the purified Pab (1:60 dilution).
- Sample type
- K562 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with EPHA10 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human testis tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
