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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V2328 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Acidic Cytokeratin Antibody
- Antibody type
- Monoclonal
- Antigen
- Solubilized keratin extract from human stratum corneum
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Rabbit, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- AE1
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Changes in keratin expression during metastatic progression of breast cancer: impact on the detection of circulating tumor cells.
AE1 cytokeratin reaction patterns in different differentiation states of squamous cell carcinoma of the esophagus.
The diagnostic utility of the keratin profiles of hepatocellular carcinoma and cholangiocarcinoma.
Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2.
Thymocyte binding to human thymic epithelial cells is inhibited by monoclonal antibodies to CD-2 and LFA-3 antigens.
Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.
Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumours using polyclonal and monoclonal antibodies.
Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms.
The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins.
Differential staining of cytoid bodies and skin-limited amyloids with monoclonal anti-keratin antibodies.
Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
Joosse SA, Hannemann J, Spötter J, Bauche A, Andreas A, Müller V, Pantel K
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Feb 15;18(4):993-1003
Clinical cancer research : an official journal of the American Association for Cancer Research 2012 Feb 15;18(4):993-1003
AE1 cytokeratin reaction patterns in different differentiation states of squamous cell carcinoma of the esophagus.
Yang K, Lipkin M
American journal of clinical pathology 1990 Sep;94(3):261-9
American journal of clinical pathology 1990 Sep;94(3):261-9
The diagnostic utility of the keratin profiles of hepatocellular carcinoma and cholangiocarcinoma.
Johnson DE, Herndier BG, Medeiros LJ, Warnke RA, Rouse RV
The American journal of surgical pathology 1988 Mar;12(3):187-97
The American journal of surgical pathology 1988 Mar;12(3):187-97
Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2.
Listrom MB, Dalton LW
American journal of clinical pathology 1987 Sep;88(3):297-301
American journal of clinical pathology 1987 Sep;88(3):297-301
Thymocyte binding to human thymic epithelial cells is inhibited by monoclonal antibodies to CD-2 and LFA-3 antigens.
Vollger LW, Tuck DT, Springer TA, Haynes BF, Singer KH
Journal of immunology (Baltimore, Md. : 1950) 1987 Jan 15;138(2):358-63
Journal of immunology (Baltimore, Md. : 1950) 1987 Jan 15;138(2):358-63
Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.
Schermer A, Galvin S, Sun TT
The Journal of cell biology 1986 Jul;103(1):49-62
The Journal of cell biology 1986 Jul;103(1):49-62
Optimal immunoreactivity of keratin proteins in formalin-fixed, paraffin-embedded tissue requires preliminary trypsinization. An immunoperoxidase study of various tumours using polyclonal and monoclonal antibodies.
Pinkus GS, O'Connor EM, Etheridge CL, Corson JM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1985 May;33(5):465-73
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1985 May;33(5):465-73
Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms.
Spagnolo DV, Michie SA, Crabtree GS, Warnke RA, Rouse RV
American journal of clinical pathology 1985 Dec;84(6):697-704
American journal of clinical pathology 1985 Dec;84(6):697-704
The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins.
Cooper D, Schermer A, Pruss R, Sun TT
Differentiation; research in biological diversity 1984;28(1):30-5
Differentiation; research in biological diversity 1984;28(1):30-5
Differential staining of cytoid bodies and skin-limited amyloids with monoclonal anti-keratin antibodies.
Eto H, Hashimoto K, Kobayashi H, Fukaya T, Matsumoto M, Sun TT
The American journal of pathology 1984 Sep;116(3):473-81
The American journal of pathology 1984 Sep;116(3):473-81
Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies.
Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT
The Journal of cell biology 1982 Nov;95(2 Pt 1):580-8
The Journal of cell biology 1982 Nov;95(2 Pt 1):580-8
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing using Acidic Cytokeratin antibody AE1.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of human skin with Acidic Cytokeratin antibody AE1.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Acidic Cytokeratin Antibody (clone AE1). Confirmation of Integrity and Purity of the Antibody.
