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LearnH00009459-M02
antibody from Novus Biologicals
Targeting: ARHGEF6
alpha-PIX, alphaPIX, Cool-2, Cool2, KIAA0006, MRX46, αPix
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00009459-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009459-M02, RRID:AB_1144806
- Product name
- Mouse Monoclonal ARHGEF6 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ARHGEF6 - Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (2E5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis.
Mazaki Y, Onodera Y, Higashi T, Horinouchi T, Oikawa T, Sabe H
Cell communication and signaling : CCS 2017 Oct 2;15(1):36
Cell communication and signaling : CCS 2017 Oct 2;15(1):36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ARHGEF6 Antibody (2E5) [H00009459-M02] - Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARHGEF6 Antibody (2E5) [H00009459-M02] - Analysis of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF6 monoclonal antibody.
