TA502483

antibody from OriGene

Targeting: UBXN2B p37

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502483

Provider

OriGene

Proper citation

OriGene Cat#TA502483, RRID:AB_11123835

Product name

UBXN2B mouse monoclonal antibody, clone OTI2E8 (formerly 2E8)

Antibody type

Monoclonal

Description

UBXN2B mouse monoclonal antibody, clone OTI2E8 (formerly 2E8)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

UBXN2B

Isotype

IgG

Antibody clone number

OTI2E8

Vial size

100 µl

Concentration

0.75 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBXN2B (RC219137, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBXN2B.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBXN2B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-UBXN2B mouse monoclonal antibody (TA502483) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBXN2B(RC219137).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC219137 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBXN2B antibody(TA502483), and then analyzed by flow cytometry.

Validation comment

FC