A305-833A-T

antibody from Invitrogen Antibodies

Targeting: MMGT1 EMC5, TMEM32

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-833A-T

Provider

Invitrogen Antibodies

Product name

MMGT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse MMGT1 by western blot. Samples: Whole cell lysate (50 µg) from A20, NIH 3T3, CT26, EL4, and RenCa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMGT1 antibody (Product # A305-833A-T; lot A305-833A-T-2) used for WB at a dilution of 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MMGT1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-7, RKO, Hep-G2, Jurkat, HEK293T, GaMG, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMGT1 antibody (Product # A305-833A-T; lot A305-833A-T-2) used for WB at a dilution of 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MMGT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMGT1 antibody A305-833A-M (lot A305-833A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MMGT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MMGT1 antibody (Product # A305-833A-T; lot A305-833A-T-2) used for IP at 100 µL per reaction. MMGT1 was also immunoprecipitated by a previous lot of this antibody (lot A305-833A-T-1). For blotting immunoprecipitated MMGT1, Product # A305-833A-T was used at a dilution of 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.