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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- H00001937-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001937-M01, RRID:AB_425410
- Product name
- EEF1G monoclonal antibody (M01), clone 3F11-1A10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant EEF1G.
- Antigen sequence
MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAP
PHFHFGQTNRTPEFLRKFPAGKVPAFEGDDGFCVF
ESNAIAYHVSNEELRGSTPEAAAQVVQWVSFADSD
IVPPASTWVFPTLGIMHHDKQATENAKEEVRRILG
LLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQV
LEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLC
EKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQ
AERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPF
AHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFD
KDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLD
KLRKNAFASVILFGTNNSSSISGVWVFRGQELAFP
LSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWE
GAFQHVGKAFNQGKIFK- Isotype
- IgG
- Antibody clone number
- 3F11-1A10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The eEF1γ subunit contacts RNA polymerase II and binds vimentin promoter region.
Corbi N, Batassa EM, Pisani C, Onori A, Di Certo MG, Strimpakos G, Fanciulli M, Mattei E, Passananti C
PloS one 2010 Dec 31;5(12):e14481
PloS one 2010 Dec 31;5(12):e14481
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in 293 ( Cat # L026V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EEF1G monoclonal antibody (M01), clone 3F11-1A10. Western Blot analysis of EEF1G expression in Raw 264.7(Cat # L024V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to EEF1G on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
