NBP1-94007
antibody from Novus Biologicals
Targeting: KNSTRN
C15orf23, FLJ14502, kinastrin, SKAP, TRAF4AF1
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-94007 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-94007, RRID:AB_11021299
- Product name
- Rabbit Polyclonal SKAP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SKAP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A proteomic study of mitotic phase-specific interactors of EB1 reveals a role for SXIP-mediated protein interactions in anaphase onset.
Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Tamura N, Simon JE, Nayak A, Shenoy R, Hiroi N, Boilot V, Funahashi A, Draviam VM
Biology open 2015 Jan 16;4(2):155-69
Biology open 2015 Jan 16;4(2):155-69
Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.
Shrestha RL, Draviam VM
Current biology : CB 2013 Aug 19;23(16):1514-26
Current biology : CB 2013 Aug 19;23(16):1514-26
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SKAP Antibody [NBP1-94007] - SKAP Antibody [NBP1-94007] - Simple Western lane view shows a specific band for SKAP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SKAP Antibody [NBP1-94007] - SKAP Antibody [NBP1-94007] - Electropherogram image(s) of corresponding Simple Western lane view. SKAP antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SKAP Antibody [NBP1-94007] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-KNSTRN antibody. Corresponding KNSTRN RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining in human testis and pancreas tissues using anti-KNSTRN antibody. Corresponding KNSTRN RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SKAP Antibody [NBP1-94007] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.