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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-121147 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPICE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: 293T, HeLa, BT-474, MCF7. The target is usually found in the following locations: Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center, spindle.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.78 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SPICE1 in various cell lines using a SPICE1 Polyclonal antibody (Product # PA5-121147) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SPICE1 in U2OS cells using a SPICE1 Polyclonal antibody (Product # PA5-121147). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPICE1 in human stomach using a Polyclonal antibody (Product # PA5-121147) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPICE1 in human stomach using a Polyclonal antibody (Product # PA5-121147) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPICE1 in human stomach using a Polyclonal antibody (Product # PA5-121147) at a dilution of 1:100.
