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antibody from Invitrogen Antibodies
Targeting: ARFGEF3
BIG3, C6orf92, dJ171N11.1, KIAA1244, PPP1R33
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-57623 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARFGEF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SLKLLKNQEA DQHSARLFIQ SLEGLLPRLL SLSNVEEVDT ALQNFASTFC SGMMHSPGFD GNSSLSFQML MNADSLYTAA HCALLLNLKL SHG
- Concentration
- 0.09 mg/mL
Submitted references In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation.
Gillingham AK, Bertram J, Begum F, Munro S
eLife 2019 Jul 11;8
eLife 2019 Jul 11;8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARFGEF3 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using an ARFGEF3 Polyclonal Antibody (Product # PA5-57623).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ARFGEF3 in human stomach tissue shows strong cytoplasmic positivity in chief cells. Samples were probed using an ARFGEF3 Polyclonal Antibody (Product # PA5-57623).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7. Validation of putative effectors for Rab2A, Rab5A and Rab9A. ( A ) Affinity chromatography of cell lysates (input) using GST-fusions to the indicated GTP-locked (QL) or GDP-locked Rabs. The bound proteins were eluted and probed with antibodies to the indicated proteins. Lysates were from HEK293 cells and probed for STAMBPL1, or from rat brain and probed for ARFGEF3. ( B ) Confocal micrographs of MEFs expressing either GFP-STAMPBL1 alone and probed for a Golgi marker, or co-expressing the mitochondrial Rab2A(Q65L)-BirA* as indicated. ( C ) As ( B ) except that the cells were expressing GFP-ARFGEF3. ( D ) Coomassie blue stained gel showing affinity chromatography of purified recombinant GFP-STAMBPL1 using GST fusions to the indicate GTP-locked (QL) or GDP-locked (SN) Rabs. The recombinant GFP-STAMBPL1 binds specifically to the GTP form of Rab2A. ( E ) Affinity chromatography of HeLa cell lysates (input) using GST-fusions to the indicated GTP-locked (QL) or GDP-locked Rabs. The bound proteins were eluted and probed with antibodies to the indicated proteins. TBCK, OSBPL9 and RELCH show specific binding to the GTP form of Rab5, with RELCH also binding to Rab11-GTP as previously reported ( Sobajima et al., 2018 ). ( F ) Confocal micrographs of cells expressing GFP-TBCK alone, or with co-expression of mitochondrial Rab5A(Q79L)-BirA* as indicated. ( G ) Coomassie blue stained gel showing affinity chromatography of lysate from E. coli expressing His 6 -Nde1 (input) using
