PA5-30700

antibody from Invitrogen Antibodies

Targeting: NXF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30700

Provider

Invitrogen Antibodies

Product name

NXF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.

Concentration

0.95 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), IMR32 (Lane 2), Hep G2 (Lane 3) and U-2 OS (Lane 4). The blot was probed with Anti-NXF3 Polyclonal Antibody (Product # PA5-30700, 1:4000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 60 kDa band corresponding to NXF3 was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NXF3 Polyclonal Antibody (Product # PA5-30700). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: IMR32. Lane C: U87-MG. Lane D: MCF-7 . 7.5% SDS PAGE. NXF3 Polyclonal Antibody (Product # PA5-30700) diluted at 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of NXF3 was achieved by transfecting U-87 MG cells with NXF3 specific siRNAs (Silencer® select Product # s31847, s224404). Western blot analysis (Fig. a) was performed using whole cell extracts from the NXF3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with NXF3 Polyclonal Antibody (Product # PA5-30700, 1:4000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NXF3.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NXF3 in paraformaldehyde-fixed MCF-7 cells using a NXF3 polyclonal antibody (Product # PA5-30700) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3 (Product # PA5-30700) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.