A305-851A-T
antibody from Invitrogen Antibodies
Targeting: LSM14B
bA11M20.3, C20orf40, FAM61B, FLJ25473, FT005, LSM13, RAP55B
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [2]
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Validation data
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- Product number
- A305-851A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LSM14B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LSM14B by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, MCF-7, U2OS, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LSM14B antibody (A305-851A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LSM14B by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, U2OS, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LSM14B antibody A305-851A-M (lot A305-851A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LSM14B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LSM14B antibody (Product # A305-851A-T; lot 2) used for IP at 6 µg per reaction. LSM14B was also immunoprecipitated by a previous lot of this antibody (Product # A305-851A-T; lot 1) and a second antibody against a different epitope of LSM14B (BL23715). For blotting immunoprecipitated LSM14B, Product # A305-851A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human LSM14B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LSM14B antibody (Product # A305-851A-T lot 2) used for IP at 6 µg per reaction. LSM14B was also immunoprecipitated by a previous lot of this antibody (A305-851A-T lot 1) and a second antibody against a different epitope of LSM14B (BL23715). For blotting immunoprecipitated LSM14B (Product # A305-851A-T was used at 1:1000 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.