GTX129298

antibody from GeneTex

Targeting: CUL4B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX129298

Provider

GeneTex

Product name

Cullin 4b antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Human DNA Ligase I Interacts with and Is Targeted for Degradation by the DCAF7 Specificity Factor of the Cul4-DDB1 Ubiquitin Ligase Complex.

Peng Z, Liao Z, Matsumoto Y, Yang A, Tomkinson AE
The Journal of biological chemistry 2016 Oct 14;291(42):21893-21902

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg Neuro2A whole cell lysate/extract B. 30 £gg C8D30 whole cell lysate/extract C. 30 £gg NIH-3T3 whole cell lysate/extract D. 30 £gg Raw 264.7 whole cell lysate/extract E. 30 £gg C2Cl2 whole cell lysate/extract5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg PC-12 whole cell lysate/extract B. 30 £gg Rat2 whole cell lysate/extract5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cullin 4b antibody detects Cullin 4b protein by western blot analysis.A. 30 £gg A375 whole cell lysate/extract 5 % SDS-PAGECullin 4b antibody (GTX129298) dilution: 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cullin 4b antibody detects Cullin 4b protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cullin 4b protein stained by Cullin 4b antibody (GTX129298) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cullin 4b antibody detects Cullin 4b protein at nucleus on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma. Cullin 4b antibody (GTX129298) dilution: 1:1000.