12916-1-AP

antibody from Proteintech Group

Targeting: CUL4B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12916-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12916-1-AP, RRID:AB_2086699

Product name

CUL4B antibody

Antibody type

Polyclonal

Description

KD/KO validated CUL4B antibody (Cat. #12916-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Schabla NM, Swanson PC
PloS one 2021;16(10):e0258683

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CUL4(high) Lung Adenocarcinomas Are Dependent on the CUL4-p21 Ubiquitin Signaling for Proliferation and Survival.

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The American journal of pathology 2021 Sep;191(9):1638-1650

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The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

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Overexpressed NEDD8 as a potential therapeutic target in esophageal squamous cell carcinoma.

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Cancer biology & medicine 2021 Mar 18;

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Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the  receptor protein of CRL2^Prame complex.

Zhang W, Li L, Cai L, Liang Y, Xu J, Liu Y, Zhou L, Ding C, Zhang Y, Zhao H, Qin J, Shao Z, Wei W, Jia L
Cell death and differentiation 2021 Jun;28(6):1926-1940

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A functional motif of long noncoding RNA Nron against osteoporosis.

Jin F, Li J, Zhang YB, Liu X, Cai M, Liu M, Li M, Ma C, Yue R, Zhu Y, Lai R, Wang Z, Ji X, Wei H, Dong J, Liu Z, Wang Y, Sun Y, Wang X
Nature communications 2021 Jun 3;12(1):3319

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DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Wang X, Wang HY, Hu GS, Tang WS, Weng L, Zhang Y, Guo H, Yao SS, Liu SY, Zhang GL, Han Y, Liu M, Zhang XD, Cen X, Shen HF, Xiao N, Liu CQ, Wang HR, Huang J, Liu W, Li P, Zhao TJ
Cell reports 2021 Jun 22;35(12):109281

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The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Tang WS, Weng L, Wang X, Liu CQ, Hu GS, Yin ST, Tao Y, Hong NN, Guo H, Liu W, Wang HR, Zhao TJ
Cell reports 2021 Jul 6;36(1):109314

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FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.

Shen JZ, Qiu Z, Wu Q, Finlay D, Garcia G, Sun D, Rantala J, Barshop W, Hope JL, Gimple RC, Sangfelt O, Bradley LM, Wohlschlegel J, Rich JN, Spruck C
Cell 2021 Jan 21;184(2):352-369.e23

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The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.

Dar AA, Sawada K, Dybas JM, Moser EK, Lewis EL, Park E, Fazelinia H, Spruce LA, Ding H, Seeholzer SH, Oliver PM
PLoS biology 2021 Feb;19(2):e3001041

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Roles of the ubiquitin ligase CUL4B and ADP-ribosyltransferase TiPARP in TCDD-induced nuclear export and proteasomal degradation of the transcription factor AHR.

Rijo MP, Diani-Moore S, Yang C, Zhou P, Rifkind AB
The Journal of biological chemistry 2021 Aug;297(2):100886

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Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.

Dong X, Han Y, Zhang E, Wang Y, Zhang P, Wang C, Zhong L, Li Q
Aging 2021 Apr 4;13(7):10603-10618

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Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.

Bayraktar G, Yuanxiang P, Confettura AD, Gomes GM, Raza SA, Stork O, Tajima S, Suetake I, Karpova A, Yildirim F, Kreutz MR
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 2020 Nov;45(12):2120-2130

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Rational discovery of molecular glue degraders via scalable chemical profiling.

Mayor-Ruiz C, Bauer S, Brand M, Kozicka Z, Siklos M, Imrichova H, Kaltheuner IH, Hahn E, Seiler K, Koren A, Petzold G, Fellner M, Bock C, Müller AC, Zuber J, Geyer M, Thomä NH, Kubicek S, Winter GE
Nature chemical biology 2020 Nov;16(11):1199-1207

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Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.

Zhou L, Zhu J, Chen W, Jiang Y, Hu T, Wang Y, Ye X, Zhan M, Ji C, Xu Z, Wang X, Gu Y, Jia L
Cell death & disease 2020 Nov 12;11(11):975

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CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.

Fu SJ, Hu MC, Peng YJ, Fang HY, Hsiao CT, Chen TY, Jeng CJ, Tang CY
Cells 2020 May 26;9(6)

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CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.

Li T, Wu S, Jia L, Cao W, Yao Y, Zhao G, Li H
Cancer biology & medicine 2020 May 15;17(2):357-370

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The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth.

Cui D, Dai X, Shu J, Ma Y, Wei D, Xiong X, Zhao Y
Cell death and differentiation 2020 Mar;27(3):1119-1133

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Effective targeting of the ubiquitin-like modifier NEDD8 for lung adenocarcinoma treatment.

Jiang Y, Cheng W, Li L, Zhou L, Liang Y, Zhang W, Chen W, Wang S, Zhao H, Chen G, Zhou W, Ji G, Zhang Y, Hoffman RM, Wang M, Jia L
Cell biology and toxicology 2020 Aug;36(4):349-364

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The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency.

Yamamoto M, Suwa Y, Sugiyama K, Okashita N, Kawaguchi M, Tani N, Matsubara K, Nakamura A, Seki Y
Journal of cell science 2020 Aug 14;133(15)

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Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes.

Yu Q, Hu Z, Shen Y, Jiang Y, Pan P, Hou T, Pan ZQ, Huang J, Sun Y
Neoplasia (New York, N.Y.) 2020 Apr;22(4):179-191

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The CRL3^BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration.

Li L, Zhang W, Liu Y, Liu X, Cai L, Kang J, Zhang Y, Chen W, Dong C, Zhang Y, Wang M, Wei W, Jia L
Signal transduction and targeted therapy 2020 Apr 24;5(1):42

---

Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.

Lai TC, Hu MC
Scientific reports 2019 Mar 28;9(1):5304

---

CUL4B promotes prostate cancer progression by forming positive feedback loop with SOX4.

Qi M, Hu J, Cui Y, Jiao M, Feng T, Li X, Pang Y, Chen X, Qin R, Su P, Zhang H, Wang Y, Gong Y, Han B
Oncogenesis 2019 Mar 14;8(3):23

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Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.

Zhou W, Ma L, Ding L, Guo Q, He Z, Yang J, Qiao H, Li L, Yang J, Yu S, Zhao L, Wang S, Liu HM, Suo Z, Zhao W
Journal of medicinal chemistry 2019 Jun 13;62(11):5382-5403

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Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.

Sun H, Zhang J, Xin S, Jiang M, Zhang J, Li Z, Cao Q, Lou H
PLoS genetics 2019 Feb;15(2):e1007685

---

The orphan nuclear receptor SHP regulates ER stress response by inhibiting XBP1s degradation.

Sun S, Kelekar S, Kliewer SA, Mangelsdorf DJ
Genes & development 2019 Aug 1;33(15-16):1083-1094

---

UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F.

Zhou W, Xu J, Tan M, Li H, Li H, Wei W, Sun Y
Molecular cell 2018 Jun 21;70(6):1008-1024.e6

---

Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.

Nagai T, Mukoyama S, Kagiwada H, Goshima N, Mizuno K
Journal of cell science 2018 Dec 12;131(24)

---

Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.

Jia L, Yan F, Cao W, Chen Z, Zheng H, Li H, Pan Y, Narula N, Ren X, Li H, Zhou P
The Journal of biological chemistry 2017 Feb 17;292(7):2966-2978

---

Sirtuin 7-dependent deacetylation of DDB1 regulates the expression of nuclear receptor TR4.

Karim MF, Yoshizawa T, Sobuz SU, Sato Y, Yamagata K
Biochemical and biophysical research communications 2017 Aug 19;490(2):423-428

---

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, Williams NS, Nijhawan D
Science (New York, N.Y.) 2017 Apr 28;356(6336)

---

Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90β.

Peng YJ, Huang JJ, Wu HH, Hsieh HY, Wu CY, Chen SC, Chen TY, Tang CY
Scientific reports 2016 Sep 1;6:32444

---

Cell Autonomous and Nonautonomous Function of CUL4B in Mouse Spermatogenesis.

Yin Y, Liu L, Yang C, Lin C, Veith GM, Wang C, Sutovsky P, Zhou P, Ma L
The Journal of biological chemistry 2016 Mar 25;291(13):6923-35

---

Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.

Eichner R, Heider M, Fernández-Sáiz V, van Bebber F, Garz AK, Lemeer S, Rudelius M, Targosz BS, Jacobs L, Knorn AM, Slawska J, Platzbecker U, Germing U, Langer C, Knop S, Einsele H, Peschel C, Haass C, Keller U, Schmid B, Götze KS, Kuster B, Bassermann F
Nature medicine 2016 Jul;22(7):735-43

---

A transcriptional target of androgen receptor, miR-421 regulates proliferation and metabolism of prostate cancer cells.

Meng D, Yang S, Wan X, Zhang Y, Huang W, Zhao P, Li T, Wang L, Huang Y, Li T, Li Y
The international journal of biochemistry & cell biology 2016 Apr;73:30-40

---

The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.

Chen YA, Peng YJ, Hu MC, Huang JJ, Chien YC, Wu JT, Chen TY, Tang CY
Scientific reports 2015 May 29;5:10667

---

The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer.

Xu J, Li L, Yu G, Ying W, Gao Q, Zhang W, Li X, Ding C, Jiang Y, Wei D, Duan S, Lei Q, Li P, Shi T, Qian X, Qin J, Jia L
Molecular & cellular proteomics : MCP 2015 Mar;14(3):499-509

---

Tumor suppressor protein Lgl mediates G1 cell cycle arrest at high cell density by forming an Lgl-VprBP-DDB1 complex.

Yamashita K, Ide M, Furukawa KT, Suzuki A, Hirano H, Ohno S
Molecular biology of the cell 2015 Jul 1;26(13):2426-38

---

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Antonioli M, Albiero F, Nazio F, Vescovo T, Perdomo AB, Corazzari M, Marsella C, Piselli P, Gretzmeier C, Dengjel J, Cecconi F, Piacentini M, Fimia GM
Developmental cell 2014 Dec 22;31(6):734-46

---

SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway.

Yoshizawa T, Karim MF, Sato Y, Senokuchi T, Miyata K, Fukuda T, Go C, Tasaki M, Uchimura K, Kadomatsu T, Tian Z, Smolka C, Sawa T, Takeya M, Tomizawa K, Ando Y, Araki E, Akaike T, Braun T, Oike Y, Bober E, Yamagata K
Cell metabolism 2014 Apr 1;19(4):712-21

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A novel function of CRL4(Cdt2): regulation of the subunit structure of DNA polymerase δ in response to DNA damage and during the S phase.

Zhang S, Zhao H, Darzynkiewicz Z, Zhou P, Zhang Z, Lee EY, Lee MY
The Journal of biological chemistry 2013 Oct 11;288(41):29550-61

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CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

Yu C, Zhang YL, Pan WW, Li XM, Wang ZW, Ge ZJ, Zhou JJ, Cang Y, Tong C, Sun QY, Fan HY
Science (New York, N.Y.) 2013 Dec 20;342(6165):1518-21

---

Cul4B regulates neural progenitor cell growth.

Liu HC, Enikolopov G, Chen Y
BMC neuroscience 2012 Sep 19;13:112

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Essential role of the CUL4B ubiquitin ligase in extra-embryonic tissue development during mouse embryogenesis.

Liu L, Yin Y, Li Y, Prevedel L, Lacy EH, Ma L, Zhou P
Cell research 2012 Aug;22(8):1258-69

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The E3 ubiquitin ligase Cullin 4A regulates meiotic progression in mouse spermatogenesis.

Yin Y, Lin C, Kim ST, Roig I, Chen H, Liu L, Veith GM, Jin RU, Keeney S, Jasin M, Moley K, Zhou P, Ma L
Developmental biology 2011 Aug 1;356(1):51-62

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Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase.

Aggarwal P, Vaites LP, Kim JK, Mellert H, Gurung B, Nakagawa H, Herlyn M, Hua X, Rustgi AK, McMahon SB, Diehl JA
Cancer cell 2010 Oct 19;18(4):329-40

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12916-1-AP(CUL4B antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 12916-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 12916-1-AP(CUL4B antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue