H00003120-M01

antibody from Abnova Corporation

Targeting: HLA-DQB2 HLA-DXB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003120-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003120-M01, RRID:AB_1136947

Product name

HLA-DQB2 monoclonal antibody (M01), clone 4C3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HLA-DQB2.

Antigen sequence

MSWKMALQIPGGFWAAAVTVMLVMLSTPVAEARDF
PKDFLVQFKGMCYFTNGTERVRGVARYIYNREEYG
RFDSDVGEFQAVTELGRSIEDWNNYKDFLEQERAA
VDKVCRHNYEAELRTTLQRQVEPTVTISPSRTEAL
NHHNLLVCSVTDFYPAQIKVQWFRNDQEETAGVVS
TSLIRNGDWTFQILVMLEITPQRGDIYTCQVEHPS
LQSPITVEWRPRGPPPAGLLH


Isotype

IgG

Antibody clone number

4C3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HLA-DQB2 expression in transfected 293T cell line by HLA-DQB2 monoclonal antibody (M01), clone 4C3.Lane 1: HLA-DQB2 transfected lysate (Predicted MW: 26.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HLA-DQB2 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol