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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-59679 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ZNF10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZNF10 Antibody [NBP2-59679] - Total protein from human kidney, testis and Ntera2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ZNF10 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZNF10 Antibody [NBP2-59679] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human kidney tissue section with ZNF10 antibody at 1:500 dilution. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This ZNF10 antibody generated primarily a cytoplasmic staining with some nuclear positivity in all the cells of tubular epithelium. The staining was observed to be relatively stronger on the lumen side of the tubules. The cytoplasmic signal is potentially arising from the newly translated pool of this transcription factor.
