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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00023500-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00023500-M01, RRID:AB_10902208
- Product name
- DAAM2 monoclonal antibody (M01), clone 1D8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant DAAM2.
- Antigen sequence
MAPRKRSHHGLGFLCCFGGSDIPEINLRDNHPLQF
MEFSSPIPNAEELNIRFAELVDELDLTDKNREAMF
ALPPEKKWQIYCSKKKVPSLTPLATSQGSWHGVAL
AALACSCIHLMFITCQPCSRCWRNNSE- Isotype
- IgG
- Antibody clone number
- 1D8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DAAM2 expression in transfected 293T cell line by DAAM2 monoclonal antibody (M01), clone 1D8.Lane 1: DAAM2 transfected lysate (Predicted MW: 14.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DAAM2 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
