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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- TA502314 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502314, RRID:AB_11126644
- Product name
- FAM40A mouse monoclonal antibody, clone OTI7G7 (formerly 7G7)
- Antibody type
- Monoclonal
- Description
- FAM40A mouse monoclonal antibody, clone OTI7G7 (formerly 7G7)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- STRIP1
- Isotype
- IgG
- Antibody clone number
- OTI7G7
- Vial size
- 100 µl
- Concentration
- 0.66 mg/ml
Submitted references STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo.
Bazzi H, Soroka E, Alcorn HL, Anderson KV
Proceedings of the National Academy of Sciences of the United States of America 2017 Dec 19;114(51):E10928-E10936
Proceedings of the National Academy of Sciences of the United States of America 2017 Dec 19;114(51):E10928-E10936
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM40A (RC210537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM40A.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FAM40A monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FAM40A mouse monoclonal antibody (TA502314) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FAM40A(RC210537).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502314)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502314)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502314)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502314)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC210537 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FAM40A antibody(TA502314), and then analyzed by flow cytometry.
- Validation comment
- FC
