NBP2-14981

antibody from Novus Biologicals

Targeting: H3-3A H3.3A, H3F3, H3F3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-14981

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Histone H3.3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extrac C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug HL-60 whole cell lysate/extract F. 30 ug NCI-H929 whole cell lysate/extract 15 % SDS-PAGE Histone H3.3 antibody dilution: 1:10000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug JC whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract 15 % SDS-PAGE Histone H3.3 antibody dilution: 1:10000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Histone H3.3 Antibody [NBP2-14981] - A. 30 ug PC-12 whole cell lysate/extract 15 % SDS-PAGE Histone H3.3 antibody dilution: 1:10000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Histone H3.3 Antibody [NBP2-14981] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H3.3 stained by Histone H3.3 antibody diluted at 1:500.