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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [6]
- Immunohistochemistry [1]
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- Product number
- GTX112955 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112955, RRID:AB_10617900
- Product name
- Histone H3.3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
Submitted references Baculovirus IE2 Interacts with Viral DNA through Daxx To Generate an Organized Nuclear Body Structure for Gene Activation in Vero Cells.
2-methoxyestradiol induces mitotic arrest, apoptosis, and synergistic cytotoxicity with arsenic trioxide in human urothelial carcinoma cells.
Wei SC, Tsai CH, Hsu WT, Chao YC
Journal of virology 2019 Apr 15;93(8)
Journal of virology 2019 Apr 15;93(8)
2-methoxyestradiol induces mitotic arrest, apoptosis, and synergistic cytotoxicity with arsenic trioxide in human urothelial carcinoma cells.
Kuo KL, Lin WC, Ho IL, Chang HC, Lee PY, Chung YT, Hsieh JT, Pu YS, Shi CS, Huang KH
PloS one 2013;8(8):e68703
PloS one 2013;8(8):e68703
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 15% SDS PAGE GTX112955 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 15% SDS PAGE GTX112955 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE GTX112955 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H3.3 antibody detects H3F3A protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extracC. 30 ?g K562 whole cell lysate/extractD. 30 ?g THP-1 whole cell lysate/extractE. 30 ?g HL-60 whole cell lysate/extract F. 30 ?g NCI-H929 whole cell lysate/extract15% SDS-PAGEHistone H3.3 antibody (GTX112955) dilution: 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H3.3 antibody detects H3F3A protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extractB. 30 ?g JC whole cell lysate/extractC. 30 ?g BCL-1 whole cell lysate/extractD. 30 ?g C2C12 whole cell lysate/extractE. 30 ?g Raw264.7 whole cell lysate/extract15% SDS-PAGEHistone H3.3 antibody (GTX112955) dilution: 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H3.3 antibody detects H3F3A protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract15% SDS-PAGEHistone H3.3 antibody (GTX112955) dilution: 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Histone H3.3 antibody detects Histone H3.3 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H3.3 antibody (GTX112955) dilution: 1:500.
