687702

antibody from BioLegend

Targeting: H3-3A H3.3A, H3F3, H3F3A

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

687702

Provider

BioLegend

Proper citation

BioLegend Cat#687702, RRID:AB_2629551

Product name

Purified anti-Histone H3.3 Phospho (Ser31)

Antibody type

Monoclonal

Antigen

Histone H3.3 S31ph peptide (24-37)(AARKSAP(phS)TGGVKK).

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

1A8G10

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were stimulated with (top) or without (bottom) nocodazole for 24 hours, then fixed and permeabilized with cold 70% ethanol, then intracellularly stained with DAPI and purified Histone H3.3 Phospho (Ser31) antibody (clone 1A8G10), followed by anti-mouse IgG PE.

Conjugate

Unconjugated