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Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-112501 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-H3K4cr Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of H3K4cr in multiple sample types using a H3K4cr polyclonal antibody (Product # PA5-112501) at a concentration of 1.2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate (treated by 30mM sodium crotonylate for 4h) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation analysis of H3K4cr in 4*10^6 Hela cells using a H3K4cr polyclonal antibody (Product #PA5-112501). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg anti-HIST1H3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
