Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-141159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DOPEY2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DOPEY2 in different tissue lysates. Samples were incubated in DOPEY2 Polyclonal antibody (Product # PA5-141159) using a dilution of 1:500. Lane 1: mouse heart; Lane 2: human heart.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DOPEY2 in D3 cells (green). Samples were incubated in DOPEY2 Polyclonal antibody (Product # PA5-141159). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DOPEY2 in paraffin-embedded mouse heart tissue. Samples were incubated in DOPEY2 Polyclonal antibody (Product # PA5-141159). Counter stained with Hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of DOPEY2 in N2A cells. Samples were incubated in DOPEY2 Polyclonal antibody (Product # PA5-141159) using a dilution of 1:100 (red) followed by Alexa Fluor 488-conjugated Goat anti-Rabbit IgG secondary antibody. Unlabeled control (cells without incubation with primary antibody; black).