Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-24862 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEZ6L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SEZ6L2 Is an Important Regulator of Drug-Resistant Cells and Tumor Spheroid Cells in Lung Adenocarcinoma.
Lee JS, Kim HY, Won B, Kang SW, Kim YN, Jang H
Biomedicines 2020 Nov 13;8(11)
Biomedicines 2020 Nov 13;8(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SE6L2 polyclonal antibody (Product # PA5-24862) in K562 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of K562 cells using a SE6L2 polyclonal antibody (Product # PA5-24862) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Seizure-related 6 homolog-like 2 (SEZ6L2) is commonly upregulated in drug-resistant cells and TS cells. ( A ) Commonly upregulated DEGs in drug-resistant cells and TS cells compared with the corresponding control cells were shown. Only three genes ( STC1 , CHPF , and SEZ6L2 ) were commonly upregulated. ( B ) The expression levels of these three genes were compared between lung adenocarcinoma (LUAD) patients and matched normal samples by the GEPIA2 webserver ( http://gepia2.cancer-pku.cn/ ). ( C ) Expression levels of SEZ6L2 in H460 parental cells and cisplatin-resistant (CR) cells were analyzed by flow cytometry (FACSVerse) using an anti-SEZ6L2 antibody. The proportion of SEZ6L2-positive cells was expressed as the mean +- standard deviation (n = 3). ** p < 0.01 relative to H460 parental cells. ( D ) Expression levels of SEZ6L2 in H460 and A549 2D- and TS cells were analyzed by flow cytometry. The proportion of SEZ6L2-positive cells was expressed as the mean +- standard deviation (n = 3). ** p < 0.01 relative to 2D-cultured cells.