TA500310

antibody from OriGene

Targeting: PROM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500310

Provider

OriGene

Proper citation

OriGene Cat#TA500310, RRID:AB_10543961

Product name

Anti-PROM2 (Prominin 2) mouse monoclonal antibody, clone OTI5E5 (formerly 5E5)

Antibody type

Monoclonal

Description

Anti-PROM2 (Prominin 2) mouse monoclonal antibody, clone OTI5E5 (formerly 5E5)

Host

Mouse

Conjugate

Unconjugated

Epitope

PROM2

Isotype

IgG

Antibody clone number

OTI5E5

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PROM2 monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PROM2 mouse monoclonal antibody (TA500310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROM2(RC211605).

Validation comment

IF

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoprecipitation(IP) of PROM2 by using TrueMab monoclonal anti-PROM2 antibodies (Negative control: IP without adding anti-PROM2 antibody.). For each experiment, 500ul of DDK tagged PROM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug

Validation comment

IP