H00004534-M01

antibody from Abnova Corporation

Targeting: MTM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004534-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004534-M01, RRID:AB_463947

Product name

MTM1 monoclonal antibody (M01), clone 1C10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant MTM1.

Antigen sequence

MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAV
PRLPGETLITDKEVIYICPFNGPIKGRVYITNYRL
YLRSLETDSSLILDVPLGVISRIEKMGGAT


Isotype

IgG

Antibody clone number

1C10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Myopathy in a woman and her daughter associated with a novel splice site MTM1 mutation.

Hedberg C, Lindberg C, Máthé G, Moslemi AR, Oldfors A
Neuromuscular disorders : NMD 2012 Mar;22(3):244-51

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10.Lane 1: MTM1 transfected lysate(69.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MTM1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol