12368-1-AP

antibody from Invitrogen Antibodies

Targeting: PINX1 FLJ20565, Gno1, LPTL, LPTS, MGC8850, Pxr1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12368-1-AP

Provider

Invitrogen Antibodies

Product name

PINX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSMLAERRR KQKWAVDPQN TAWSNDDSKF GQRMLEKMGW SKGKGLGAQE HGATDHIKVQ VKNNHLGLGA TINNEDNWIA HQDDFNQLLA ELNTCHGQET TDSSDKKEKK SFSLEEKSKI SKNRVHYMKF TKGKDLSSRS KTDLDCIFGK RQSKKTPEGD ASPSTPEENE TTTTSAFTIQ EYFAKRMAAL KNKPQVPVPG SDISETQVER KRGKKINKEA TGKDVESYLQ PKAKRHTEGK PERAEAQERV AKKKSAPAEE QLRGPCWDQS SKASAQDAGD HVQPPEGRDF TLKPKKRRGK KKLQKPVEIA EDATLEETLV KKKKKKDSK (1-328 aa encoded by BC015479 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12368-1-AP (PINX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12368-1-AP (PINX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 12368-1-AP ( PINX1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 12368-1-AP ( PINX1 Antibody) at dilution of 1:50 (under 40x lens).