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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-27581 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPHKAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, MCF-7, SK-N-SH, IMR32, SK-N-AS.
- Concentration
- 1 mg/mL
Submitted references Mitochondrial A-kinase anchoring proteins in cardiac ventricular myocytes.
Sherpa RT, Fiore C, Moshal KS, Wadsworth A, Rudokas MW, Agarwal SR, Harvey RD
Physiological reports 2021 Sep;9(17):e15015
Physiological reports 2021 Sep;9(17):e15015
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPHKAP using 30 µg of IMR32 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SPHKAP polyclonal antibody (Product # PA5-27581) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SPHKAP was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SPHKAP Polyclonal Antibody (Product # PA5-27581) at a dilution of 1:1,000.
