HPA048168

antibody from Atlas Antibodies

Targeting: SPHKAP SKIP

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA048168

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA048168, RRID:AB_2680296

Product name

Anti-SPHKAP

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AQSTLQTKHPDIYCITDFAEELADTVVSMATEIAA
ICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLKR
KKESQGSGTAVRKHKPPRLSEIKRKTDEHP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using Anti-SPHKAP antibody. Corresponding SPHKAP RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows low expression as expected.

Sample type

HUMAN