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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [17]
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- Product number
- UM800041 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800041, RRID:AB_2629153
- Product name
- HMBS mouse monoclonal antibody,clone UMAB144
- Antibody type
- Monoclonal
- Description
- HMBS mouse monoclonal antibody,clone UMAB144
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HMBS
- Isotype
- IgG
- Antibody clone number
- UMAB144
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of KM12 cell lysate (35ug) by using anti-HMBS monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-HMBS mouse monoclonal antibody (UM800041, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-HMBSmouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on tonsil germinal and non-germinal centers. Some weak nuclear stain was also observed.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on spleen.
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Weak nuclear staining is seen on tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
- Validation comment
- IHC
