H00003145-M01

antibody from Abnova Corporation

Targeting: HMBS PBGD, PORC, UPS

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003145-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003145-M01, RRID:AB_606390

Product name

HMBS monoclonal antibody (M01), clone 3E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant HMBS.

Antigen sequence

MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQT
DSVVATLKASYPGLQFEIIAMSTTGDKIPDTALSK
IGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLP
PGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEK
SVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLR
KLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEE
CMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLL
RCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTG
GVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQL
VGITARNIPRGPQLAAQNLGISLANLLLSKGAKNI
LDVARQLNDAH


Isotype

IgG

Antibody clone number

3E8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HMBS is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol