- PA1-29421 - Invitrogen Antibodies TRPV1 antibody

Submitted references Yellow Chaste Weed and Its Components, Apigenin and Galangin, Affect Proliferation and Oxidative Stress in Blue Light-Irradiated HaCaT Cells.

Park JY, Park SH, Oh SW, Kwon K, Yu E, Choi S, Yang S, Han SB, Jung K, Song M, Cho JY, Lee J
Nutrients 2022 Mar 13;14(6)

Dose-dependent immunomodulatory effects of bortezomib in experimental autoimmune neuritis.

Klimas R, Sgodzai M, Motte J, Mohamad N, Renk P, Blusch A, Grüter T, Pedreiturria X, Gobrecht P, Fischer D, Schneider-Gold C, Reinacher-Schick A, Tannapfel A, Yoon MS, Gold R, Pitarokoili K
Brain communications 2021;3(4):fcab238

Intrathecal injection of TRPV1 shRNA leads to increases in blood pressure in rats.

Yu SQ, Wang DH
Acta physiologica (Oxford, England) 2011 Sep;203(1):139-47

Enhanced salt sensitivity following shRNA silencing of neuronal TRPV1 in rat spinal cord.

Yu SQ, Wang DH
Acta pharmacologica Sinica 2011 Jun;32(6):845-52

Brain-derived neurotrophic factor redistribution in the dorsal root ganglia correlates with neuropathic pain inhibition after resiniferatoxin treatment.

Tender GC, Li YY, Cui JG
The spine journal : official journal of the North American Spine Society 2010 Aug;10(8):715-20

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: HEK293 cells expressing combinations of TRPV1, p75, and trkA, as indicated in the figure, were treated with either NGF or vehicle and then immunoprecipitated with anti-trkA (lanes 1-7) or anti-TRPV1 (lanes 8-13) antibodies. Lanes 1-7 were separated from lanes 8-13 and the membranes were probed with anti-trkA antibody and TRPV1 Polyclonal Antibody (Product # PA1-29421), respectively (top). Segments of the membrane were then reapposed for imaging. The membranes were then stripped and reprobed with anti-phosphotyrosine antibody (bottom).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of dorsal root ganglion using TRPV1 Polyclonal Antibody (Product # PA1-29421).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of rat dorsal root ganglion using TRPV1 Polyclonal Antibody (Product # PA1-29421). Some DRG neurons express GFP (because they were infected by intrathecal administration of AAV2-5 carrying GFP). Cyan: Primary antibody. Red: IB4.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Frozen) analysis of rat DRG tissue using (Product # PA1-29421) TRPV1 Polyclonal Antibody. Green: Primary antibody. Red: NeuN. Dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Western blot analysis showing TRPV1 expression in the dorsal horn of the spinal cord (T8-L3), DRG (T8-L3), and mesenteric arteries of rats fed a NS or HS diet 3 d after control or TRPV1 shRNA treatment. The left, middle, and right panels indicate representative Western blots, quantification results (beta-actin arbitrary units), and the percentage change of TRPV1 expression of TRPV1 shRNA from that of corresponding control shRNA, respectively. Values are expressed as mean+-SEM ( n =5). b P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 YCW extract increased cell proliferation via calcium dependent-TRPV1 signaling pathways in blue light irradiated HaCaT cells. ( A ) CellTiter-Glo (r) 2.0 assay of YCW extract (0.01-0.1%) pretreated with blue light-irradiated HaCaT cells. YCW extract was pretreated 24 h before irradiation of blue light (13.68 J/cm 2 ), and the same process was repeated on the next day. After 24 h incubation, an equal volume of CellTiter-Glo reagent was added to the cells. ( B ) Cell proliferation effect of YCW extract (0.01-0.1%) on blue light-irradiated HaCaT cells was measured using BrdU ELISA assay. Data are presented as the mean +- SEM of four independent experiments. Statistical significance of differences among the groups was assessed by one-way analysis of variance (ANOVA), followed by Tukey's multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. control group. * p < 0.05 vs. control, *** p < 0.005 vs. control, # p < 0.05 vs. blue light-irradiated group (B.L), ## p < 0.01 vs. B.L. ### p < 0.005 vs. B.L. ( C ) TRPV1 expression levels were determined by Western blotting at 24 h incubation after, pretreatment with YCW extract (0.1%) for and 2-day repetitive blue light irradiation (30 min, 76 W/m 2 ). ( D ) Phosphorylated-TRPV1 expression levels were determined by Western blotting at 90 min incubation after, 1 h YCW extract (0.1%) pretreatment and blue light irradiation (30 min, 76 W/m 2 ). The total proteins were extracted from the cells immediately after e

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Apigenin and galangin reduced TRPV1 expression and its phosphorylation in blue light-irradiated HaCaT cells. ( A , B ) TRPV1 expression ( A ), and its phosphorylation levels ( B ) were determined by Western blotting. ( A ) Cells were incubated with apigenin (10, 20, 30 muM) and galangin (1, 10, 20 muM) for 24 h and were then irradiated with blue light (30 min, 76 W/m 2 ). After 24 h incubation, the cells were subjected to the same process twice and were finally subjected to Western blot analysis. ( B ) Phosphorylation levels of TRPV1 were determined by Western blotting at 30-, 60-, and 90-min incubation after pretreatment with apigenin (30 muM) or galangin (20 muM) for 1 h and subsequent blue light-irradiation (30 min, 76 W/m 2 ). The total proteins were extracted from the cells immediately after experimental conditions, and beta-actin was used as a loading control. ( C , D ) Apigenin and galangin inhibited blue light-induced calcium influx in HaCaT cells. ( C ) Ca 2+ influx changes of blue light and with apigenin (10, 20, 30 muM) pretreatment, ( D ) with galangin (1, 10, 20 muM) pretreatment by Fluo-4 NW assay. Cells were pretreated with apigenin (10, 20, 30 muM) and galangin (1, 10, 20 muM) for 1 h and irradiated with blue light (10 min, 76 W/m 2 ). After the irradiation, fluorescence intensities were measured immediately over a certain period.

PA1-29421

Antibody data