Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- ALR-003-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CysLTR1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute T-cell leukemia Jurkat (lanes 3 and 6) human cell lysates: - 1,2,3. Anti-CysLTR1 (extracellular) Antibody (#ALR-003), (1:200).4,5,6. Anti-CysLTR1 (extracellular) Antibody , preincubated with CysLTR1 (extracellular) Blocking Peptide (#BLP-LR003).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute T-cell leukemia Jurkat (lanes 3 and 6) human cell lysates: - 1,2,3. Anti-CysLTR1 (extracellular) Antibody (#ALR-003), (1:200).4,5,6. Anti-CysLTR1 (extracellular) Antibody , preincubated with CysLTR1 (extracellular) Blocking Peptide (#BLP-LR003).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of CysLTR1 in live intact THP-1 (acute monocytic leukemia) cells: - (black) Cells + goat- Anti-rabbit-Cy5. (green) Cells + Anti-CysLTR1 (extracellular) Antibody (#ALR-003), (1:50) + goat- Anti-rabbit-Cy5.