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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 603-820 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myosin Heavy Chain, Cardiac Monoclonal Antibody (9G11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive control: Cardiac muscle
- Antibody clone number
- 9G11
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: The whole cell lysate derived from rat cardiac extract was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Mouse anti – Myosin Heavy Chain (Cat#603-820) antibody at 1:500. An major immunoreactive band around ~230 kDa was observed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry: Human Tonsil (FFPE) stained with Mouse anti-Myosin Heavy Chain (Cat# 603-820) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
