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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP10357c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP10357c, RRID:AB_10821960
- Product name
- SPDYE3 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SPDYE3 Antibody (Center) (Cat. #AP10357c) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the SPDYE3 antibody detected the SPDYE3 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SPDYE3 antibody (Center) (Cat. #AP10357c) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYE3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SPDYE3 Antibody (Center) (Cat. #AP10357c) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
