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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP54010PU-N - Provider product page
- Provider
- OriGene
- Product name
- SPDYE3 (Center) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- SPDYE3 (Center) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SPDYE3
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of SPDYE3 Antibody ??in A375 cell line lysates (35ug/lane). This demonstrates the SPDYE3 antibody detected the SPDYE3 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human cervical tissue stained with SPDYE3 Antibody ??followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPDYE3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A375 cells using SPDYE3 Antibody ??(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
