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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-22050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX3Y Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HepG2, mouse brain, rat testis, Jurkat, Raji, NCI-H929.
- Concentration
- 0.89 mg/mL
Submitted references Cellular proteins associated with the interior and exterior of vesicular stomatitis virus virions.
Moerdyk-Schauwecker M, Hwang SI, Grdzelishvili VZ
PloS one 2014;9(8):e104688
PloS one 2014;9(8):e104688
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DDX3Y using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate and C) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a DDX3Y polyclonal antibody (Product # PA5-22050) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX3Y Polyclonal Antibody (Product # PA5-22050). Sample (30 µg of whole cell lysate). A: H1299. B: Hep G2. 7.5% SDS PAGE. DDX3Y Polyclonal Antibody (Product # PA5-22050) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX3Y Polyclonal Antibody (Product # PA5-22050). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. DDX3Y Polyclonal Antibody (Product # PA5-22050) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDX3Y Polyclonal Antibody detects DDX3 protein by Western blot analysis. A. 50 µg Rat testis lysate/extract.7.5 % SDS-PAGE. DDX3Y Polyclonal Antibody (Product # PA5-22050) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX3Y Polyclonal Antibody (Product # PA5-22050). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3Y Polyclonal Antibody (Product # PA5-22050) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX3Y Polyclonal Antibody (Product # PA5-22050). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3Y Polyclonal Antibody (Product # PA5-22050) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDX3Y in paraformaldehyde-fixed HeLa cells using a DDX3Y polyclonal antibody (Product # PA5-22050) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3 antibody (Product # PA5-22050) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Confirmation of host protein incorporation in virion preparations. Protein lysates from uninfected (BHK) and VSV infected (BHK+VSV) BHK-21 cells were separated by SDS-PAGE along with total protein from whole virions, proteinase K (ProK) treated virions, and viral ribonucleoprotein complexes (RNP). Loading of the virion samples was confirmed by Ponceau S staining of the membrane. The position of the viral glycoprotein (G), nucleocapsid protein (N), phosphoprotein (P), and matrix protein (M) is indicated. Western blots using primary antibodies against coiled-coil and C2 domain-containing protein 1A (Cc2d1a), protein tyrosine phosphatase type IVA 2 (Ptp4a2), putative ATP-dependent RNA helicase Pl10 (D1pas1), proto-oncogene tyrosine-protein kinase Yes (Yes1), casein kinase I isoform alpha (Csnk1a1), heat shock cognate 71 kDa protein kDa protein (Hspa8) and E3 ubiquitin-protein ligase Itchy (ITCH), were conducted as indicated to determine the presence of the host proteins. Approximate molecular mass of the proteins is given on the left.
