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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1789 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-28B/IFN-lambda 3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects IL-28B/IFN-lambda 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human IL-28A is observed. In Western blots, approximately 35% cross-reactivity (reducing conditions) and approximately 10% cross-reactivity (non-reducing conditions) with recombinant mouse IL-28A is observed. Neutralizes 100% of recombinant mouse IL-28A.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Expression and purification of biologically active bovine Interferon λ3 (IL28B) in Pichia pastoris.
Barathiraja S, Gangadhara PAV, Umapathi V, Dechamma HJ, Reddy GR
Protein expression and purification 2018 May;145:14-18
Protein expression and purification 2018 May;145:14-18
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- IL-28B/IFN-lambda 3 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL-28B/IFN-lambda 3 Antibody. Recombinant Mouse IL-28B/IFN-lambda 3 (Catalog # 1789-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IL-28B/ IFN-lambda 3 (400 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-28B/IFN-lambda 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1789). The ND50 is typically 2-10 µg/mL.
