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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB5259 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-28B/IFN-lambda 3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-28B/IFN-lambda 3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-28A, rhIL-29, or recombinant mouse IL-28 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAN28264- Isotype
- IgG
- Antibody clone number
- 567143
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Macrophage migration inhibitory factor enhances influenza-associated mortality in mice.
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.
Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
Smith CA, Tyrell DJ, Kulkarni UA, Wood S, Leng L, Zemans RL, Bucala R, Goldstein DR
JCI insight 2019 Jul 11;4(13)
JCI insight 2019 Jul 11;4(13)
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.
Corry J, Arora N, Good CA, Sadovsky Y, Coyne CB
Proceedings of the National Academy of Sciences of the United States of America 2017 Aug 29;114(35):9433-9438
Proceedings of the National Academy of Sciences of the United States of America 2017 Aug 29;114(35):9433-9438
Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells.
Stone AE, Giugliano S, Schnell G, Cheng L, Leahy KF, Golden-Mason L, Gale M Jr, Rosen HR
PLoS pathogens 2013;9(4):e1003316
PLoS pathogens 2013;9(4):e1003316
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-28B/IFN-lambda 3 by Western Blot. Western blot shows lysates of human monocytes treated (+) with 10 ng/mL of rhIFN-alpha for 24 hours and then further stimulated with 10 µg/mL of poly I:C for 48 hours. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-28B/IFN-lambda 3 Monoclonal Antibody (Catalog # MAB5259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-28B/IFN-lambda 3 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
