PAB28421

antibody from Abnova Corporation

Targeting: PNN memA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28421

Provider

Abnova Corporation

Product name

PNN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PNN.

Antigen sequence

EQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYI
PGRMCPATQKLIEESQRKMNALFEGRRIEFAEQIN
KMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQ
REEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDA
EKE


Isotype

IgG

Storage

Store at 4 °C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: RT-4, lane 2: EFO-21 and lane 3: A-431 cell lysates using PNN polyclonal antibody (Cat # PAB28421).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of A-431 cell line with PNN polyclonal antibody (Cat # PAB28421) shows positivity in nuclei but not nucleoli. Fixation/Permeabilization: PFA/Triton X-100

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human duodenum with PNN polyclonal antibody (Cat # PAB28421) shows strong nuclear positivity in glandular cells.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)