Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 18266-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pinin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EKQQDSQPE EVMDVLEMVE NVKHVIADQE VMETNRVESV EPSENEASKE LEPEMEFEIE PDKECKSLSP GKENVSALDM EKESEEKEEK ESEPQPEPVA QPQPQSQPQL QLQSQSQPVL QSQPPSQPED LSLAVLQPTP QVTQEQGHLL PERKDFPVES VKLTEVPVEP VLTVHPESKS KTKTRSRSRG RARNKTSKSR SRSSSSSSSS SSSTSSSSGS SSSSGSSSSR SSSSSSSSTS GSSSRDSSSS TSSSSESRSR SRGRGHNRDR KHRRSVDRKR RDTSGLERSH KSSKGGSSRD TKGSKDKNSR SDRKRSISES SRSGKRSSRS ERDRKSDRKD KRR (376-717 aa encoded by BC062602)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.26 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18266-1-AP ( Pinin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (Product # 18266-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PNN antibody 18266-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 18266-1-AP (Pinin antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 18266-1-AP (Pinin antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Pinin (IP:18266-1-AP, 4ug; Detection:18266-1-AP 1:300) with HeLa cells lysate 520ug.