HPA059449

antibody from Atlas Antibodies

Targeting: DOK7 C4orf25, Dok-7, FLJ33718, FLJ39137

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA059449

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA059449, RRID:AB_2684023

Product name

Anti-DOK7

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SSLDVWRATDELGSLLSLPAAGAPEPSLCTCLPGT
VEYQVPTSLRAHYDTPRSLCLAPRDHS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line MCF-7 and human cell line SK-MEL-30.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-DOK7 antibody HPA059449 (A) shows similar pattern to independent antibody HPA062780 (B).

Antibody #2 product nr

HPA062780

Antibody provider

Atlas Antibodies

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Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Sample type

HUMAN