Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-32122 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLRT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FLRT2 using 30 µg U87-MG whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a FLRT2 polyclonal antibody (Product # PA5-32122) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FLRT2 Polyclonal Antibody (Product # PA5-32122). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FLRT2 Polyclonal Antibody (Product # PA5-32122) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FLRT2 Polyclonal Antibody (Product # PA5-32122). U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FLRT2 Polyclonal Antibody (Product # PA5-32122) diluted at 1:2,500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using FLRT2 (Product # PA5-32122) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.