GTX116609

antibody from GeneTex

Targeting: PPP2R2D B55D, B55delta, MDS026

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX116609

Provider

GeneTex

Proper citation

GeneTex Cat#GTX116609, RRID:AB_10615217

Product name

PPP2R2D antibody [N2C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Therapeutic relevance of the PP2A-B55 inhibitory kinase MASTL/Greatwall in breast cancer.

Álvarez-Fernández M, Sanz-Flores M, Sanz-Castillo B, Salazar-Roa M, Partida D, Zapatero-Solana E, Ali HR, Manchado E, Lowe S, VanArsdale T, Shields D, Caldas C, Quintela-Fandino M, Malumbres M
Cell death and differentiation 2018 May;25(5):828-840

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Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d.

Joseph BK, Liu HY, Francisco J, Pandya D, Donigan M, Gallo-Ebert C, Giordano C, Bata A, Nickels JT Jr
The Journal of biological chemistry 2015 Apr 24;290(17):10588-98

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Activation of p107 by fibroblast growth factor, which is essential for chondrocyte cell cycle exit, is mediated by the protein phosphatase 2A/B55α holoenzyme.

Kurimchak A, Haines DS, Garriga J, Wu S, De Luca F, Sweredoski MJ, Deshaies RJ, Hess S, Graña X
Molecular and cellular biology 2013 Aug;33(16):3330-42

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX116609 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A:IMR32 10% SDS PAGE GTX116609 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.