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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00003859-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003859-D01P, RRID:AB_10642329
- Product name
- KRT12 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human KRT12 protein.
- Antigen sequence
MDLSNNTMSLSVRTPGLSRRLSSQSVIGRPRGMSA
SSVGSGYGGSAFGFGASCGGGFSAASMFGSSSGFG
GGSGSSMAGGLGAGYGRALGGGSFGGLGMGFGGSP
GGGSLGILSGNDGGLLSGSEKETMQNLNDRLASYL
DKVRALEEANTELENKIREWYETRGTGTADASQSD
YSKYYPLIEDLRNKIISASIGNAQLLLQIDNARLA
AEDFRMKYENELALRQGVEADINGLRRVLDELTLT
RTDLEMQIESLNEELAYMKKNHEDELQSFRVGGPG
EVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKD
AEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRR
AFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLS
QVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKA
RLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQA
QSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEI
EELM- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KRT12 expression in transfected 293T cell line (H00003859-T01) by KRT12 MaxPab polyclonal antibody.Lane 1: KRT12 transfected lysate(54.34 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to KRT12 on HeLa cell. [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
