MA5-42701

antibody from Invitrogen Antibodies

Targeting: KRT12 K12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42701

Provider

Invitrogen Antibodies

Product name

Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody (2M1K10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

2M1K10

Vial size

100 µL

Concentration

0.33 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Cytokeratin 12 in Rat eye using a Cytokeratin 12 Recombinant Monoclonal antibody (Product # MA5-42701) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cytokeratin 12 in Mouse eye using a Cytokeratin 12 Recombinant Monoclonal antibody (Product # MA5-42701). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Cytokeratin 12 in Mouse eye using a Cytokeratin 12 Recombinant Monoclonal antibody (Product # MA5-42701). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Cytokeratin 12 in mouse cornea using a Recombinant Monoclonal antibody (Product # MA5-42701) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Cytokeratin 12 in mouse cornea using a Recombinant Monoclonal antibody (Product # MA5-42701) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..