Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29522 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 31 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa.
- Concentration
- 0.85 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 31 Polyclonal Antibody (Product # PA5-29522). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. Cytokeratin 31 Polyclonal Antibody (Product # PA5-29522) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cytokeratin 31 Polyclonal Antibody detects KRT31 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT31 protein stained by Cytokeratin 31 Polyclonal Antibody (Product # PA5-29522) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Gastric ca, using Cytokeratin 31 (Product # PA5-29522) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
