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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA1-342 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP2A6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- PA1-342 detects cytochrome P450 (P450) 2A6 from human, mouse and rat samples. This antibody detects, to a lesser extent, other P450 isoforms.
Submitted references Expression and activity of cytochromes P450 2E1, 2A, and 2B in the mouse ovary: the effect of 4-vinylcyclohexene and its diepoxide metabolite.
Cannady EA, Dyer CA, Christian PJ, Sipes IG, Hoyer PB
Toxicological sciences : an official journal of the Society of Toxicology 2003 Jun;73(2):423-30
Toxicological sciences : an official journal of the Society of Toxicology 2003 Jun;73(2):423-30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot detection of cytochrome P450 2A6 from rat liver extract using Product # PA1-342.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and Mouse Pancreas (Lane 2). The blots were probed with Anti-Cytochrome P450 2A6 Rabbit polyclonal Antibody (Product # PA1-342, 1:1000-1:5000 dilution dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 56 kDa band corresponding to Cytochrome P450 2A6 was observed in Hep G2 but not in Mouse Pancreas. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
