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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-28534 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Collagen IV Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Microporous scaffolds support assembly and differentiation of pancreatic progenitors into β-cell clusters.
Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study.
Youngblood RL, Sampson JP, Lebioda KR, Shea LD
Acta biomaterialia 2019 Sep 15;96:111-122
Acta biomaterialia 2019 Sep 15;96:111-122
Inflammatory-Driven Angiogenesis in Bone Augmentation with Bovine Hydroxyapatite, B-Tricalcium Phosphate, and Bioglasses: A Comparative Study.
Anghelescu VM, Neculae I, Dincă O, Vlădan C, Socoliuc C, Cioplea M, Nichita L, Popp C, Zurac S, Bucur A
Journal of immunology research 2018;2018:9349207
Journal of immunology research 2018;2018:9349207
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Collagen IV was performed in human kidney, glomeruli and liver tissues using Collagen IV Polyclonal Antibody (Product # PA1-28534) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 12 TCP 100x collagen IV stain showing moderate fibrosis.
