NBP1-85639

antibody from Novus Biologicals

Targeting: UBQLN2 Chap1, CHAP1/DSK2, Dsk2, LIC-2, N4BP4, PLIC-2, PLIC2, RIHFB2157

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85639

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85639, RRID:AB_11032720

Product name

Rabbit Polyclonal Ubiquilin 2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Ubiquilin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Mutant UBQLN2 promotes toxicity by modulating intrinsic self-assembly.

Sharkey LM, Safren N, Pithadia AS, Gerson JE, Dulchavsky M, Fischer S, Patel R, Lantis G, Ashraf N, Kim JH, Meliki A, Minakawa EN, Barmada SJ, Ivanova MI, Paulson HL
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 30;115(44):E10495-E10504

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Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.

Yau RG, Doerner K, Castellanos ER, Haakonsen DL, Werner A, Wang N, Yang XW, Martinez-Martin N, Matsumoto ML, Dixit VM, Rape M
Cell 2017 Nov 2;171(4):918-933.e20

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Differential recruitment of UBQLN2 to nuclear inclusions in the polyglutamine diseases HD and SCA3.

Zeng L, Wang B, Merillat SA, Minakawa EN, Perkins MD, Ramani B, Tallaksen-Greene SJ, Costa MDC, Albin RL, Paulson HL
Neurobiology of disease 2015 Oct;82:281-288

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Viral expression of ALS-linked ubiquilin-2 mutants causes inclusion pathology and behavioral deficits in mice.

Ceballos-Diaz C, Rosario AM, Park HJ, Chakrabarty P, Sacino A, Cruz PE, Siemienski Z, Lara N, Moran C, Ravelo N, Golde TE, McFarland NR
Molecular neurodegeneration 2015 Jul 8;10:25

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Tau pathology in frontotemporal lobar degeneration with C9ORF72 hexanucleotide repeat expansion.

Bieniek KF, Murray ME, Rutherford NJ, Castanedes-Casey M, DeJesus-Hernandez M, Liesinger AM, Baker MC, Boylan KB, Rademakers R, Dickson DW
Acta neuropathologica 2013 Feb;125(2):289-302

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Ubiquilin 2 Antibody [NBP1-85639] - Simple Western lane view shows a specific band for Ubiquilin 2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Ubiquilin 2 Antibody [NBP1-85639] - Electropherogram image(s) of corresponding Simple Western lane view. Ubiquilin 2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ubiquilin 2 Antibody [NBP1-85639] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-UBQLN2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human placenta shows moderate to strong cytoplasmic positivity in cytotrophoblast.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebellum shows moderate to strong cytoplasmic and nuclear positivity in Purkinje cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons and glial cells.