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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PAB0186 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0186, RRID:AB_1018872
- Product name
- HIST3H2BB polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 111-125 of human HIST3H2BB.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Zebrafish
- Host
- Rabbit
- Antigen sequence
AVSEGTKAVTKYTSS- Vial size
- 100 µg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of fractionated Cos-7 cells using HIST3H2BB polyclonal antibody (Cat # PAB0186) . Cos-7 cells were fractionated into nuclear & cytosolic/membrane fractions. Approximately ~15ug of each fraction was separated on SDS PAGE. Lane 1, Nuclear fraction. Lane 2, Cytosolic / membrane fraction.
- Validation comment
- Western Blot (Cell lysate)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HIST3H2BB in human PBMC lysate with HIST3H2BB polyclonal antibody (Cat # PAB0186) .
- Validation comment
- Western Blot (Cell lysate)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HIST3H2BB using HIST3H2BB polyclonal antibody (Cat # PAB0186) at 1 : 250 in nuclear and cytoplasmic fractions. Data courtesy of Dr. Jerry Yin, University of Wisconsin-Madison.
- Validation comment
- Western Blot (Tissue lysate)
